Dissecting the regulatory mechanisms of alternative polyadenylation and nuclear retention of mRNAs by SR proteins
SR proteins are central RNA-binding proteins that accompany mRNAs throughout their journey from transcription in the nucleus to translation in the cytoplasm. They thereby hold the potential to link subsequent RNA processing steps for nuclear surveillance. However, their functions beyond splicing remain poorly studied. Here, we will elucidate the molecular mechanisms underlying the roles of SR proteins in alternative polyadenylation and regulated RNA export. We will study the physiological consequences of these regulatory functions in the context of neuronal differentiation.structure thus reflects its functional importance. We will use this structural conservation to identify previously unknown cis-regulatory elements and establish a high-throughput screen for the parallel analysis of thousands of conserved 3’UTR structures in different cell types and under different stress conditions. The importance of trans-acting factors known to recognize mRNA structures will be tested globally for all functional motifs, and the best candidates characterized in detail. More
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Michaela Müller-McNicoll: Biologicum, Campus Riedberg
Gebäudeteil B, 1.OG
Max-von-Laue-Straße 13
60438 Frankfurt am Main, Germany
Kathi Zarnack: Buchmann Institute for Molecular Life Sciences Max-von-Laue-Str. 15 60438 Frankfurt am Main, Germany
Co-Workers on RNA
Hannah Eich
PhD. Student (Müller-McNicoll group)
m6A RNA modification, APA, translation, RNA binding proteins
Email: hannaheich@web.de
Phone: +49 69 798 42092
Benjamin Arnold
PhD. Student (Müller-McNicoll group)
RNA binding proteins, rapid protein degradation, membrane-less organelles
Email: Arnold@bio.uni-frankfurt.de
Phone: +49 69 798 42092
Clara Hernandez
PhD. Student (Müller-McNicoll group)
ArcRNAs, hypoxia, membrane-less organelles, bioinformatics
Email: HernandezCanas@em.uni-frankfurt.de
Phone: +49 69 798 42092
Mario Keller
Postdoc (Zarnack group)
APA, alternative splicing, bioinformatics, RNA binding proteins, iCLIP